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dacF [2020-05-19 16:08:37]
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dacF [2020-05-19 16:08:37]

penicillin-binding protein I, D-alanyl-D-alanine carboxypeptidase
locus
BSU_23480
pI
9.91
mw
43.13 kDa
protein length
389 aa Sequence Blast
gene length
1170 bp Sequence Blast
function
carboxypeptidase
product
penicillin-binding protein I, D-alanyl-D-alanine carboxypeptidase
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.7|Penicillin-binding proteins]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
  • [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

    • Gene

    Coordinates
    2,445,094 → 2,446,263

    The protein

    Catalyzed reaction/ biological activity

  • modifies degree of cross-linking of glycan strands in peptidoglycan
  • Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine (according to UniProt)

    • Protein family

  • [SW|Peptidase S11 family] (according to UniProt)

    • Paralogous protein(s)

  • [protein|7C3081BC8D416127A881627EB56C0628359111CF|DacA], [protein|76825D77907E8CE47B17ED56D7E2A348B1ADA5EC|DacB]

    • Structure

  • [PDB|4K91] (from Pseudomonas aeruginosa, 38% identity) [pubmed|23629710]

    • [SW|Localization]

  • secreted (via signal peptide) (according to UniProt)

    • Expression and Regulation

    Operons

    genes
    [gene|F7AD78F9AB98A150E8CDFC1D01A9F938FF9F8BD1|dacF]-[gene|3936E91C062074BFE4284B54A8CC33F35F94F5EE|spoIIAA]-[gene|C8B867758330355F44C63CC284DC7AA3061FB9F1|spoIIAB]-[gene|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, [Pubmed|15699190], in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon]
  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]

    • regulatory mechanism

  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

    • regulation

  • ''[SW|spoIIAA]'': expressed early during sporulation
  • strongly repressed in the presence of salt (1.2 M NaCl) [pubmed|32419322]
    • view in new tab

    Biological materials

    Mutant

  • BKE23480 (Δ[gene|F7AD78F9AB98A150E8CDFC1D01A9F938FF9F8BD1|dacF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCAAAAGCCCTCCATT, downstream forward: _UP4_TAATTATGCCGAATGACCAC
  • BKK23480 (Δ[gene|F7AD78F9AB98A150E8CDFC1D01A9F938FF9F8BD1|dacF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23480 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTCAAAAGCCCTCCATT, downstream forward: _UP4_TAATTATGCCGAATGACCAC

    • References

    Reviews

  • 18266855

    • Original publications

  • 16497325,8936302,9864321,12107147,20817675,22123250,23629710